Skeletal Muscle Cells Assay

Alignment, organization and shape standardization

4DCELL DEVICE

Micropatterns

 

READ-OUTS

Alignment and organization of skeletal muscle cells

 

STANDARD CULTURE LIMITATION

In vivo, cells are highly organized to form structures, tissues, and organs – and that structure helps determine function. Conventional 2D cell culture conditions – in which cells are given a boundless surface on which to grow in any orientation – don’t reflect the cell’s natural microenvironment.

 

SKELETAL MUSCLE CELLS ASSAY

When cultured in a substrate with line adhesive cues, skeletal muscle cells get organized and acquire an elongated shape. Using 4Dcell assay, skeletal myoblasts are more easily differentiated.

 

EXAMPLE

Micropatterned culture with myoblasts [1]

C2C12 cells are seeded on micropatterned lines to induce differentiation

Micropatterned culture with myoblasts

Immunofluorescence of cells [1]

Nuclei in blue and Myosine Heavy Chain (MHC) in green in C2C12 cells cultured on micropatterned lines. Organization and alignment of cells are required to induce cell contractility. By improving the cell alignment, cell differentiation leading to cell contractility will occur rapidly.

immunofluorescence of cells (MHC and nuclei)